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Usda Aphis Ghost Dna Detection on botanical science

botanical science with usda aphis ghost dna detection

What's Happening

Standard qPCR tests used by USDA APHIS cannot distinguish between live pathogens and dead DNA fragments from previously infected tissue. This 'Ghost DNA' detection leads to the destruction of healthy, sterile plants that test positive for pathogens that no longer exist. PMAxx™-based viable qPCR solves this by selectively amplifying only live cells.

How to Fix It

  1. 1

    Request PMAxx viable qPCR testing for high-value imports

  2. 2

    Obtain phytosanitary certificates with viable-cell testing specifications

  3. 3

    Document sterile tissue culture origin to support pathogen-free claims

  4. 4

    Pre-treat samples with PMAxx dye before qPCR to eliminate ghost signals

How to Prevent It

Work with exporters who use PMAxx-based viable qPCR protocols; maintain sterile tissue culture documentation throughout shipping chain; understand that standard qPCR will detect dead DNA fragments from any previous contamination.

Related Problems

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Frequently Asked Questions

What causes usda aphis ghost dna detection on my plant?
Standard qPCR tests used by USDA APHIS cannot distinguish between live pathogens and dead DNA fragments from previously infected tissue. This 'Ghost DNA' detection leads to the destruction of healthy,...
How do I fix usda aphis ghost dna detection?
Request PMAxx viable qPCR testing for high-value imports. Obtain phytosanitary certificates with viable-cell testing specifications.
How do I prevent usda aphis ghost dna detection from happening again?
Work with exporters who use PMAxx-based viable qPCR protocols; maintain sterile tissue culture documentation throughout shipping chain; understand that standard qPCR will detect dead DNA fragments fro...