Dracaena: propagation
Quick answer
propagation for Dracaena: Surface sterilization of Dracaena explants is critical because endophytic contaminants and epiphytic microorganisms rapidly colonize meristematic tissue, causing culture death within 72 hours of inoculation
Start here:
- 1. 1. Collect terminal buds from healthy donor plants, rinse under running water for 5 minutes
- 2. 2. Prepare Clorox (commercial bleach, 5.25% sodium hypochlorite) solutions at 20%, 30%, 40%, 50% concentrations
Why propagation matters for Dracaena
Dracaena spp.
Surface sterilization of Dracaena explants is critical because endophytic contaminants and epiphytic microorganisms rapidly colonize meristematic tissue, causing culture death within 72 hours of inoculation
How to handle propagation on Dracaena
- 1
1. Collect terminal buds from healthy donor plants, rinse under running water for 5 minutes
- 2
2. Prepare Clorox (commercial bleach, 5.25% sodium hypochlorite) solutions at 20%, 30%, 40%, 50% concentrations
- 3
3. Immerse explants in selected Clorox solution for exactly 20 minutes with gentle agitation
- 4
4. Rinse explants 3 times with sterile distilled water under aseptic conditions
- 5
5. Inoculate onto MS medium at 3/4 strength, free of growth regulators
- 6
6. Incubate at 25±2°C, 70% RH, 3000 lux light intensity for 16h/day photoperiod
- 7
7. Record survival percentage at 3 weeks post-inoculation
Keeping Dracaena on track
Always work under laminar flow hood, use sterile distilled water rinses, and autoclave all glassware at 121°C for 25 minutes before filling with medium