Ficus Carica: in vitro propagation failure
Quick answer
in vitro propagation failure for Ficus Carica: Sucrose concentration in MS medium directly controls carbon source availability for in vitro plantlets — low sucrose (1%) favors shoot meristem activity and leaf expansion while high sucrose (5%) drives root initiation and elongation through increased osmotic potential and carbohydrate reserves (Elazab & Shaaban 2015)
Start here:
- 1. 1. Collect 0.5cm shoot tips with 4-5 leaf primordia from Aboudy fig cultivar
- 2. 2. Surface sterilize 10 min in 2% sodium hypochlorite, rinse 4× sterile water
Why in vitro propagation failure matters for Ficus Carica
Sucrose concentration in MS medium directly controls carbon source availability for in vitro plantlets — low sucrose (1%) favors shoot meristem activity and leaf expansion while high sucrose (5%) drives root initiation and elongation through increased osmotic potential and carbohydrate reserves (Elazab & Shaaban 2015)
How to handle in vitro propagation failure on Ficus Carica
- 1
1. Collect 0.5cm shoot tips with 4-5 leaf primordia from Aboudy fig cultivar
- 2
2. Surface sterilize 10 min in 2% sodium hypochlorite, rinse 4× sterile water
- 3
3. Culture on MS + 0.5 mg/L BAP + 2 mg/L GA3 + 0.2 mg/L IBA + 0.1 g/L myo-inositol + 3% sucrose, pH 5.8
- 4
4. Multiply at 25°C, 16h photoperiod, 2200 lux, 75% humidity for 8 weeks
- 5
5. For MAXIMUM ROOT GROWTH: transfer to MS + 0.1 mg/L IBA + 5% sucrose → yields 28.67 roots, 2.49cm length
- 6
6. For MAXIMUM SHOOT GROWTH: use 1% sucrose → yields 3.6cm shoot length, 8.7 leaves
- 7
7. Root growth scoring: 1=poor(0.5-1cm), 2=medium(1-2cm), 3=good(2-3cm), 4=very good(>3cm)
Keeping Ficus Carica on track
For rooting: use 5% sucrose in MS+0.1mg/L IBA. For shoot multiplication: use 1% sucrose. Standard 3% sucrose is a compromise that optimizes neither parameter optimally.