null on Pothos
Quick answer
If your Pothos has null, the most likely cause is: The Murashige and Skoog (MS) medium, developed in 1962, remains the gold standard basal medium for plant tissue culture due to its balanced macro and micronutrient composition that supports optimal cell growth and morphogenesis for most species.
Start here:
- 1. 1. Dissolve MS basal salts in distilled water at standard concentrations
- 2. 2. Add 30 g/L sucrose as carbon source and 0.5-1 mg/L myo-inositol
Why Pothos gets null
Epipremnum aureum
The Murashige and Skoog (MS) medium, developed in 1962, remains the gold standard basal medium for plant tissue culture due to its balanced macro and micronutrient composition that supports optimal cell growth and morphogenesis for most species.
How to reverse null on Pothos
- 1
1. Dissolve MS basal salts in distilled water at standard concentrations
- 2
2. Add 30 g/L sucrose as carbon source and 0.5-1 mg/L myo-inositol
- 3
3. Adjust pH to 5.7-5.8 with NaOH/KOH before adding gelling agent
- 4
4. Autoclave at 121°C for 20 minutes; add heat-labile components filter-sterilized after cooling
Keep null off this Pothos
Prepare fresh MS medium every 2-3 weeks. Store at 4°C in darkness as vitamins degrade. Discard if precipitation or discoloration occurs.
Other Pothos problems
Same Problem on Other Plants
Go Deeper
This is covered in-depth in the pothos Mastery Pack — structured modules with video walkthroughs, advanced protocols, and rescue timelines.
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