null on Snake-Plant
Quick answer
If your Snake-Plant has null, the most likely cause is: Somatic embryogenesis mimics zygotic embryo development but from somatic cells, requiring 2,4-D in the induction phase and removal or reduction of auxin for maturation and germination of the embryos.
Start here:
- 1. 1. Induce embryogenic callus on MS medium with 2,4-D (1-2 mg/L) and casein hydrolysate (500 mg/L)
- 2. 2. Transfer to liquid medium with reduced 2,4-D (0.5 mg/L) for embryo development
Why Snake-Plant gets null
Somatic embryogenesis mimics zygotic embryo development but from somatic cells, requiring 2,4-D in the induction phase and removal or reduction of auxin for maturation and germination of the embryos.
How to reverse null on Snake-Plant
- 1
1. Induce embryogenic callus on MS medium with 2,4-D (1-2 mg/L) and casein hydrolysate (500 mg/L)
- 2
2. Transfer to liquid medium with reduced 2,4-D (0.5 mg/L) for embryo development
- 3
3. Mature embryos on hormone-free medium with increased sucrose (5-8%)
- 4
4. Germinate on half-strength MS medium without growth regulators
Keep null off this Snake-Plant
Use immature zygotic embryos or young leaf tissue as explants for higher embryogenic potential. Avoid prolonged 2,4-D exposure which can cause somaclonal variation.
Other Snake-Plant problems
Same Problem on Other Plants
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