bioactive compound production on Ficus
Quick answer
If your Ficus has bioactive compound production, the most likely cause is: Phytochemical elicitation using methyl jasmonate (MeJA) upregulates phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) genes, directing carbon flux toward the phenylpropanoid pathway, producing gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, cinnamic acid, rutin, quercetin, and apigenin in Ficus carica hairy root cultures.
Start here:
- 1. 1. Induce hairy root cultures using Agrobacterium rhizogenes strain A7 for 100% transformation rate
- 2. 2. Culture in Woody Plant Medium (WPM) at 25°C with 16:8 light:dark photoperiod
Why Ficus gets bioactive compound production
Ficus spp.
Phytochemical elicitation using methyl jasmonate (MeJA) upregulates phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) genes, directing carbon flux toward the phenylpropanoid pathway, producing gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, cinnamic acid, rutin, quercetin, and apigenin in Ficus carica hairy root cultures.
How to reverse bioactive compound production on Ficus
- 1
1. Induce hairy root cultures using Agrobacterium rhizogenes strain A7 for 100% transformation rate
- 2
2. Culture in Woody Plant Medium (WPM) at 25°C with 16:8 light:dark photoperiod
- 3
3. Apply methyl jasmonate (MeJA) elicitation at 100-200 μmol/L concentration
- 4
4. Harvest hairy roots at 21-28 days post-elicitation for peak phenolic content
- 5
5. Extract using 80% ethanol aqueous solution and concentrate under reduced pressure
Keep bioactive compound production off this Ficus
For maximum phenolic compound yield in Ficus carica: apply 100-200 μmol/L MeJA elicitation at 7-10 days post-inoculation; for maximum flavonoid content, use 200 μmol/L MeJA for Siah cultivar.