hairy root culture on Ficus
Quick answer
If your Ficus has hairy root culture, the most likely cause is: Agrobacterium rhizogenes-mediated transformation transfers the rolB oncogene into Ficus carica genome, inducing autonomous hairy root formation that produces phenolic compounds (gallic acid 5848 μg/g, caffeic acid 508 μg/g, rutin 43.5 μg/g, quercetin 341 μg/g) at levels significantly exceeding those of intact plant tissue.
Start here:
- 1. 1. Prepare Ficus carica Sabz and Siah cultivar explants (3-week-old shoots and leaves)
- 2. 2. Inoculate with Agrobacterium rhizogenes strain A7 (OD600 0.5-0.8)
Why Ficus gets hairy root culture
Ficus spp.
Agrobacterium rhizogenes-mediated transformation transfers the rolB oncogene into Ficus carica genome, inducing autonomous hairy root formation that produces phenolic compounds (gallic acid 5848 μg/g, caffeic acid 508 μg/g, rutin 43.5 μg/g, quercetin 341 μg/g) at levels significantly exceeding those of intact plant tissue.
How to reverse hairy root culture on Ficus
- 1
1. Prepare Ficus carica Sabz and Siah cultivar explants (3-week-old shoots and leaves)
- 2
2. Inoculate with Agrobacterium rhizogenes strain A7 (OD600 0.5-0.8)
- 3
3. Co-cultivate on Woody Plant Medium (WPM) for 48 hours at 22°C in darkness
- 4
4. Transfer to hormone-free WPM containing 500 mg/L cefotaxime for selection
- 5
5. Subculture hairy roots every 14 days, confirming transformation via GFP or GUS assay
- 6
6. Elicit with 100-200 μmol/L MeJA at day 14 of subculture for enhanced phenolic production
Keep hairy root culture off this Ficus
Use Agrobacterium rhizogenes strain A7 for optimal transformation efficiency; maintain sterile in vitro conditions with 25°C ± 2°C temperature to prevent culture contamination and maintain secondary metabolite productivity.