micropropagation on Ficus
Quick answer
If your Ficus has micropropagation, the most likely cause is: In Ficus carica micropropagation, three sequential stages are required: establishment (MS + BAP + GA3 + IBA, 8 weeks at 25±1°C), proliferation (MS + 3% sucrose), and rooting (MS + 0.1 mg/L NAA + variable sucrose). Sucrose concentration at 5% in the rooting stage yields 28.67 roots versus 9.7 at 1%, demonstrating the critical role of sugar availability during this developmental transition.
Start here:
- 1. Stage 1 (Establishment): MS + 0.5 mg/L BAP + 2 mg/L GA3 + 0.2 mg/L IBA + 30 g/L sucrose, 8 weeks
- 2. Stage 2 (Proliferation): Transfer to fresh MS + BAP medium at 3% sucrose
Why Ficus gets micropropagation
Ficus spp.
In Ficus carica micropropagation, three sequential stages are required: establishment (MS + BAP + GA3 + IBA, 8 weeks at 25±1°C), proliferation (MS + 3% sucrose), and rooting (MS + 0.1 mg/L NAA + variable sucrose). Sucrose concentration at 5% in the rooting stage yields 28.67 roots versus 9.7 at 1%, demonstrating the critical role of sugar availability during this developmental transition.
How to reverse micropropagation on Ficus
- 1
Stage 1 (Establishment): MS + 0.5 mg/L BAP + 2 mg/L GA3 + 0.2 mg/L IBA + 30 g/L sucrose, 8 weeks
- 2
Stage 2 (Proliferation): Transfer to fresh MS + BAP medium at 3% sucrose
- 3
Stage 3 (Rooting): MS + 0.1 mg/L NAA + 30-50 g/L sucrose depending on goal
- 4
Scoring: 1=poor (<1 cm root), 2=medium (1-2 cm), 3=good (2-3 cm), 4=very good (>3 cm)
Keep micropropagation off this Ficus
Maintain sterile technique: 2% sodium hypochlorite for 10 min, rinse 4x with sterile water, autoclave at 121°C for 20 min.