tissue culture carbon source on Ficus
Quick answer
If your Ficus has tissue culture carbon source, the most likely cause is: In in vitro conditions, sucrose is the essential carbon source because photosynthesis is limited by low light intensity (2200 lux), low CO₂, and absence of soil organic matter — the plant cannot fix sufficient carbon autotrophically and relies entirely on exogenous sugar for energy and biosynthesis.
Start here:
- 1. Add sucrose at 20-30 g/L for standard micropropagation
- 2. Use 50 g/L (5%) sucrose when root development is the primary goal
Why Ficus gets tissue culture carbon source
Ficus spp.
In in vitro conditions, sucrose is the essential carbon source because photosynthesis is limited by low light intensity (2200 lux), low CO₂, and absence of soil organic matter — the plant cannot fix sufficient carbon autotrophically and relies entirely on exogenous sugar for energy and biosynthesis.
How to reverse tissue culture carbon source on Ficus
- 1
Add sucrose at 20-30 g/L for standard micropropagation
- 2
Use 50 g/L (5%) sucrose when root development is the primary goal
- 3
Autoclave sucrose-containing medium at 121°C for 20 min at pH 5.8
Keep tissue culture carbon source off this Ficus
Standard range is 20-30 g/L (2-3%); do not exceed 5% (50 g/L) as high sucrose causes carbohydrate accumulation that inhibits photosynthesis.