null on Monstera
Quick answer
If your Monstera has null, the most likely cause is: Meristem culture exploits the fact that viral particles rarely penetrate the apical meristem dome due to limited vascular connections, making the shoot tip (0.1-1 mm) a source of virus-free plant material for indexing and elimination.
Start here:
- 1. 1. Excise shoot tips of 0.1-1 mm including 1-2 leaf primordia
- 2. 2. Surface sterilize with 0.1% mercuric chloride for 5 minutes
Why Monstera gets null
Meristem culture exploits the fact that viral particles rarely penetrate the apical meristem dome due to limited vascular connections, making the shoot tip (0.1-1 mm) a source of virus-free plant material for indexing and elimination.
How to reverse null on Monstera
- 1
1. Excise shoot tips of 0.1-1 mm including 1-2 leaf primordia
- 2
2. Surface sterilize with 0.1% mercuric chloride for 5 minutes
- 3
3. Culture on MS medium with reduced cytokinin (0.05 mg/L BAP)
- 4
4. Root in vitro or graft to sterile seedling rootstock for establishment
Keep null off this Monstera
Select meristems from disease-tested mother plants. Combine with thermotherapy or chemotherapy (ribavirin) for viruses that do penetrate meristems.