null on Philodendron
Quick answer
If your Philodendron has null, the most likely cause is: Callus induction requires exposing explants to high auxin (2,4-D 1-5 mg/L) which disrupts organized growth and triggers dedifferentiation of cells back to a meristematic state capable of rapid division.
Start here:
- 1. 1. Surface sterilize explants (leaf segments, stem nodes) with 1% NaOCl for 15 minutes
- 2. 2. Culture on MS medium with 2,4-D (2 mg/L) and kinetin (0.5 mg/L)
Why Philodendron gets null
Philodendron spp.
Callus induction requires exposing explants to high auxin (2,4-D 1-5 mg/L) which disrupts organized growth and triggers dedifferentiation of cells back to a meristematic state capable of rapid division.
How to reverse null on Philodendron
- 1
1. Surface sterilize explants (leaf segments, stem nodes) with 1% NaOCl for 15 minutes
- 2
2. Culture on MS medium with 2,4-D (2 mg/L) and kinetin (0.5 mg/L)
- 3
3. Maintain at 25°C in dark for 4-6 weeks until callus forms
- 4
4. Subculture every 2-3 weeks to fresh medium to prevent browning
Keep null off this Philodendron
Use young tissue with high mitotic activity. Maintain dark conditions initially as light can inhibit callus formation in some species.
Other Philodendron problems
Same Problem on Other Plants
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